Comparative Silver Staining Patterns of Water Buffalo, Goat, and Pig Spermatozoa

Analysis of chromatin integrity and DNA damage of buffalo spermatozoa

This study was conducted to determine chromatin integrity and DNA damage by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and DNA damage. A significant variation was found in semen parameters after thawing. Highly sig...

Effects of In Vitro Zinc Sulphate Additive to The Semen Extender on Water Buffalo (Bubalusbubalis) Spermatozoa before and after Freezing

Silver Staining of Alzheimer's Disease.

Paraffin sections were deparaffinized and hydrated to distilled water, then placed in 20% silver nitrate and kept in 60°C for 15 minutes, then rinsed in distilled water. Then sections were treated with formalin solution containing ammoniacal silver solution for about 15 min, followed by rinsing and placing tissue in sodium thiosulfate solution for 2 minutes and washing in tap water. The section...

Formation of Hydrogen Peroxide by Chilled Goat Spermatozoa and the Effects of Dead Spermatozoa on Motility Characteristics

In this study, the formation of hydrogen peroxide (H2O2) by chilled goat spermatozoa was measured. Furthermore, the effects of dead spermatozoa on motility characteristics were studied. Fresh collected ejaculates from five Shami bucks were centrifuged and virtually all seminal plasma was removed. A part of the collected spermatozoa was killed by two ways: the first by repe...

Analysis of chromatin integrity and DNA damage of buffalo spermatozoa.

This study was conducted to determine chromatin integrity and DNA damage by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and DNA damage. A significant variation was found in semen parameters after thawing. Highly sig...